If you've been smoking weed for a while, you've probably heard of THC. But have you ever heard of THCA? A unique cannabinoids that. THCA Cannabinoid Review - Say Hello to the Non-psychoactive THC In other words, the medicinal properties of weed are the result of the chemical. What is THCA? Say Hello to the 'Other' THC May 6, | MarijuanaBreak Federal Shutdown Means Cheap Marijuana for Government Employees.
to THCA? Hello Other the is THC… What Say
There you have it, a non-psychoactive and super-therapeutic cannabis drink. You could also use it as a garnish for dinner entrees, or infuse it in salad dressing. By now you might be wondering how THCA actually works. Basically, this is the receptor that makes mint feel cold. It is therefore thought that by interacting with these receptors, the THCA produces therapeutic benefits.
When it comes to the comparison of raw cannabis versus smoking it, it is thought that raw cannabis is a lot more potent and therefore more effective. William Courtney, the consumption of raw cannabis means that you ingest over 1, times more beneficial cannabinoid acids than you would if you were to smoke or vaporize dried buds.
Although there is still much research to be done on THCA and its medicinal properties, preclinical studies have uncovered a wide variety of potential uses for this compound. The ECS has been described as the largest neurotransmitter network in the body. Evidence has shown THCA to work as a sleep promoter, powerful pain-reliever, anxiety-reducer, and anti-inflammatory.
Essentially THCA has all of the same medicinal benefits of regular cannabis but without producing any kind of high. While, as we have already mentioned, research into THCA is still in its infancy, the studies that have been done have delivered exceptionally intriguing and promising results. The interesting thing is that while most anti-inflammatory medications have severe side effects, cannabinoids are much safer. Other preclinical studies have shown that THCA may be even better than THC when it comes to treating the nausea effects of chemotherapy.
It seems that some of these effects were through CB1 activation, but THCA also helped through another unknown mechanism as well. It is also well known that many cannabinoids have neuroprotective properties, and THCA is no exception.
Another study found that THCA protects dopaminergic neurons against cell death. On a molecule level, they are almost identical. In other words, if you smoke it or vape it, it will be broken down into THC which will result in mind-altering, psychoactive properties.
If you use THCA without heating it, by means of drinking it in a tea or as a smoothie, you can get its medicinal effects without getting high. Lots of great info here, thanks for posting. THCA is more accurate though because you decarb it on the rig. I wish the acidic cannabinoids were more recognized medicianlly, so it would legitimize patients growing many plants for juicing.
Juicing takes a lot of plant material. The best results for pain in my opinion are through edibles. I think there is a happy medium. Taking clones and growing most to veg only really shortens the lifecycle 1. Personally though, I think being a heavy defoliator during veg and initial flowering would be a happy compromise between juicing material and happy buds. Posted using Partiko iOS, join the beta testing program here.
Another factor to recognize with juicing, is that since it is not psychoactive, you can consume not only more thca but other cannabinoids that are present in lower quantities in the plant. I would like to chat with you regarding a CBD opportunity.
My email is dr thedrevil. I've heard about juicing cannabis, but I didn't know it had a mint quality to it. This post is very good, i like this and my work is very close with the determination of THC en biological samples.
If you want to juice raw plant leaves, get friendly with a home grower. Many growers defoliate regularly to increase bud yield and would love to give the leaves away instead of having them grow to waste.
See what I did there? To listen to the audio version of this article click on the play image. Brought to you by tts. If you find it useful please consider upvote this reply. What type of grinding did you use to grind the samples to the fine powder prior to further extraction? We used a simple, small Bamix food processing wand with the dry ingredients attachment to do grinding of the medical marijuana, but this is not something for larger scale work! Great question, and I should have been more explicit.
It brings up an interesting point though in that we often increase extraction efficiency for pesticide residue testing by pre-wetting our dry samples even marijuana with water prior to doing extraction. Thanks for the question! Running comparisons of ETOH. Water is not an issue when using a.
Using ETOH is best case however expensive by comparison. Sample prep for flowers is done with good results by hand selecting pieces working from the apical meristems down pulling off three to 6 bractiols at a time and droping them into a pre tared 40 ml VOA vile. Weigh out in the vial mg of flowers in this manner,. Sample prep for hashis, ground to small particles, followed by extraction and heating. Use a clean wooden chopstick, and disturb, crush and remain large particulates, return VOA vial cap shake and then let settle befor injection.
Edibles start at mg, 1G an up, extract with solvent disturb with a chopstick add MFSTA and pyridine ul each to a mixing vial and inject into extract to derrivatize triglycerides that are found in most edibles.
These triglycerides can bind to the sim dist collumn destroying its seperation efficiency. Thanks for your comments, Chris! Good point about minimal to no impact of water on your 0. Are you using split injection?
Many thanks for your sample prep education, Chris! Interesting approach to preserving column efficiency and lifetime by derivatization of triglycerides! Or would it make a negligible difference? But back to the subject… Pentane is extremely non-polar, as you note in your comment, so I expect its performance would be similar to hexane, which gave the lowest recovery for the THC acid converted to THC upon hot split GC injection. One knock against pentane in this case, independent of extraction efficiency, is its volatility.
Similarly to methylene chloride, it has such a high vapor pressure that it tends to evaporate while sitting in the vial on the autosampler tray and that could change the determined result. In this situation, an internal standard of some sort should be added to improve quantitative results.
Have you tried splitting your sample and running on HPLC? I was very pleased to see your blogs on cannabis testing. We do the same and have of course the same problem of the very difficult matrix. So the solvent experiments looks nice, at least the colour of the extract is different, with acetonitril being a possible good choice. However, the colour does not always is a good indicator for the problems during analysis dirt on the GC -liner and peaks that can potentially mask the components you are looking for.
It would be nice that life is so simple! Can you say something about the effect of the different solvents on the troubles in the GC linear and do you have examples of the GC TIC chromatograms from the different solvents? That would be very much appreciated. Thank you so much for your kind comments on our work. Dirt, however, is deadly on gas chromatography, as it can quickly foul the liner and lead to suppression of analyte response, or in some cases, degradation of analyte e.
DDT is a good example. The high flow through the inlet will help suppress many inlet issues that can arise for this type of work. I hope this was helpful, and again, thank you for your comments. If you have any suggestions for other cannabis testing experiments, please let me know.
Your excellent research has inspired me to ask a few questions. Have you considered running an HPLC test if you have access to eliminate the thermal degradation that occurs due to the heating process involved in GC? That way you can run an analysis using the different solvents and compare the ratio of Cannabinoid acid levels to the Cannabinoids present after each solvent extraction. Did you ever get results for using water as the solvent hot degrees C or cold degrees C?
Have you tried employing acidic water pH 2. Also, have you considered running a double solvent extraction to minimize the levels of chlorophyl present in the extract? For example, extracting with methanol followed by hexane to remove the chlorophyl.
Last question, have you considered employing different thermodynamic properties to the experiment. Thanks for your outstanding research, it is greatly appreciated! LC is certainly the way to go for analyzing the cannabinoid acids e. What would you expect? The suggestion for doing back extraction with hexane to remove chlorophyll is also very interesting to me, and I will try that next time to determine if that can provide some basic sample cleanup that will result in longer uptime for the GC system.
CBN start falling off in response after sample analyses and those responses almost always come back after changing the GC inlet liner and bottom seal, and trimming a loop about 0.
I think hot solvent extraction, or any other vigorous means of extraction e. Many thanks for them. Is there a non alcoholic solvent which can be used to get an efficient extraction?
If so please name it. Methanol and isopropanol were the only alcohols used in the study posted. In general, all of these solvents gave relatively good extraction efficiency based on cannabinoid determinations via GC. Hi, Based on the bar graph showing all of the weight percentages between 3.
The quick answer is no, and is highly driven by the fact that Pennsylvania, where Restek resides, is not a medical cannabis State. We worked under the supervision of the police and Penn State University to do our pesticides and cannabinoids work on confiscated illicit marijuana, but by necessity and law!
Caveat above aside, I expect similar extraction performance for higher and lower cannabinoid content, at least on plant material, especially flowers. However, I do think it would be of great value to the industry eventually to do an exhaustive-extraction study with various solvents across a range of materials.
For example, when determining pesticides in food, some labs use kg amounts of strawberries, homogenized, to produce a representative smaller sample for extraction, i. My question for the medical cannabis community, at least for plant material, is how representative is the potency value obtained by using a small amount for extraction? And, I assume with edibles production the dose is more accurately controlled anyway. Looking for data on quick wash with ethanol, efficiency of quick wash of decarboxylated material to extract active ingredients without water soluble components.
Another question, do you know the amount of or concentration odd chlorophyll present in these samples? Lastly, if you were going to continue to remove the chlorophyll, have you thought of an additional filtering setup or quecher.
Generally, as long as your sample representativeness requirements are met and your limits of detection for components of interest can be met, you can minimize the sample extracted to keep the GC system up longer.
Also, using split injection with the proper inlet liner is a great way to extend column lifetime and system uptime, too. I do not have any data for proof ethanol as an extraction solvent for cannabis for potency determinations. Not sure what you mean about water-soluble components, but would expect ethanol to extract polar compounds which generally have higher water solubilities relatively efficiently.
This is the thread I have been looking for! I repeat steps 3 and 4 twice more for a final average potency, and this value is used on our purchase contracts.
What is THCA? Say Hello to the “Other” THC…
If you can wrap your head around THC and THCA, you will have a strong grasp Well, hello there! THCA – Tetrahydrocannabinolic acid (say that 5 times fast!) high) different cannabinoids isolated from cannabis, exhibiting varied effects. Raw cannabis belongs to the realm of superfoods alongside avocados, kale, and greek yogurt; they help fight arthritis, chronic pain, and other maladies. You've heard of THC, and while they may sound similar, THCA has very different properties. Unlike THC, THCA is a non-intoxicating.